8CLZ
Crystal structure of Rhizobium etli constitutive L-asparaginase ReAIV (monoclinic form R4mC-2)
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-11-23 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9770 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 96.245, 85.298, 82.946 |
Unit cell angles | 90.00, 109.18, 90.00 |
Refinement procedure
Resolution | 78.340 - 1.501 |
Rwork | 0.253 |
R-free | 0.30240 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.479 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 78.340 | 1.590 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.088 | 0.849 |
Rmeas | 0.107 | 1.044 |
Number of reflections | 100056 | 16066 |
<I/σ(I)> | 6.6 | 1.12 |
Completeness [%] | 99.1 | |
Redundancy | 2.99 | |
CC(1/2) | 0.994 | 0.517 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M ammonium formate, 20 % (w/v) PEG 3350, and 0.2% lauryldimethylamine oxide (LDAO) |