8CLT
Zearalenone lactonase of Rhodococcus erythropolis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-07-26 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.976250 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 64.467, 121.803, 172.356 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.530 - 1.460 |
| R-factor | 0.165 |
| Rwork | 0.164 |
| R-free | 0.19880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.014 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1-4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.530 | 1.512 |
| High resolution limit [Å] | 1.460 | 1.460 |
| Rmerge | 0.101 | 4.677 |
| Rmeas | 0.111 | 5.527 |
| Rpim | 0.044 | 2.872 |
| Number of reflections | 114638 | 9488 |
| <I/σ(I)> | 10.2 | 0.18 |
| Completeness [%] | 93.0 | 39.33 |
| Redundancy | 6 | 0.18 |
| CC(1/2) | 0.999 | 0.109 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 295.15 | 0.2 M NaCl, 0.1 M BIS-TRIS 5.5 pH, 25% w/v PEG 3350 |
