8CLM
1.80 A crystal structure of RNA/2'-O-methyl-RNA heteroduplex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | RIGAKU PhotonJet-S |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-04-12 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.54184 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 30.103, 49.303, 55.868 |
| Unit cell angles | 90.00, 97.15, 90.00 |
Refinement procedure
| Resolution | 19.011 - 1.800 |
| Rwork | 0.188 |
| R-free | 0.25610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.952 |
| Data reduction software | CrysalisPro (41.100a) |
| Data scaling software | Aimless (0.7.7) |
| Phasing software | MOLREP (11.7.03) |
| Refinement software | REFMAC (5.8.0403) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.011 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.081 | 0.636 |
| Rmeas | 0.094 | 0.747 |
| Rpim | 0.046 | 0.382 |
| Number of reflections | 15124 | 868 |
| <I/σ(I)> | 11.7 | 2.1 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 4.2 | 3.6 |
| CC(1/2) | 0.998 | 0.817 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 1 mM duplex, 1.4 M Li2SO4, 50 mM sodium cacodylate, 1 mM spermine |
