8CKQ

Crystal structure of maize cytokinin oxidase/dehydrogenase 4 (CKO/CKX4) in complex with inhibitor 2-[(3,5-dichlorophenyl)carbamoylamino]benzamide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-05
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.980109
Spacegroup name	P 43 21 2
Unit cell lengths	79.470, 79.470, 203.570
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.190 - 2.000
R-factor	0.197
Rwork	0.196
R-free	0.21900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	0.980
Data reduction software	XDS (BUILT=20190315)
Data scaling software	XDS (BUILT=20190315)
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.800	2.120
High resolution limit [Å]	2.000	2.000
Rmeas	0.133	2.764
Rpim	0.028	0.570
Number of reflections	45188	7101
<I/σ(I)>	15.4	1.3
Completeness [%]	99.8	99
Redundancy	22.2	22.4
CC(1/2)	0.998	0.710

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	25% MPD in 50 mM HEPES, pH 7.5, 1.4 mM inhibitor and protein at 9.7 mg ml-1 concentration in the starting drop