8CJV

Structure of bovine CD46 ectodomain (SCR 1-4)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-02
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.976
Spacegroup name	P 41 21 2
Unit cell lengths	129.731, 129.731, 120.193
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	88.170 - 2.840
R-factor	0.2543
Rwork	0.253
R-free	0.28550
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.840
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	91.730	3.240
High resolution limit [Å]	2.840	2.840
Rmerge	0.487	2.225
Rmeas	0.497	2.278
Rpim	0.097	0.473
Number of reflections	10884	544
<I/σ(I)>	7.1	1.8
Completeness [%]	93.3
Redundancy	25.6	20.5
CC(1/2)	0.998	0.630

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.4	293	10.5mg/ml in 0.1M sodium cacodylate tetrahydrate pH 6.4, 14% PEG smear broad, 0.2M CaCl2, 5% glycerol .