8CIA
Crystal structure of the kelch domain of human KLHL20
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-05-21 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 152.928, 152.928, 195.819 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 71.230 - 3.720 |
| R-factor | 0.2287 |
| Rwork | 0.226 |
| R-free | 0.28670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.760 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHENIX (1.19.2_4158) |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 132.440 | 3.780 |
| High resolution limit [Å] | 3.720 | 3.720 |
| Number of reflections | 27259 | 1405 |
| <I/σ(I)> | 8 | 1.3 |
| Completeness [%] | 95.8 | |
| Redundancy | 20.4 | |
| CC(1/2) | 1.000 | 0.600 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 20% PEG2000MME, 0.2M Trimethylamine N-Oxide, 0.1M Tris pH 8.5 |
