8CI3

Structure of bovine CD46 ectodomain (SCR 1-2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-25
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.976
Spacegroup name	P 41 21 2
Unit cell lengths	91.090, 91.090, 121.123
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	72.800 - 2.330
R-factor	0.2283
Rwork	0.227
R-free	0.25570
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	0.890
Data scaling software	DIALS
Phasing software	PHASER (1.18.2_3874)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	72.800	2.413
High resolution limit [Å]	2.330	2.330
Rmerge	0.234	3.599
Rmeas	0.238	3.671
Rpim	0.047	0.718
Number of reflections	22417	2168
<I/σ(I)>	10.19	1.12
Completeness [%]	99.4	99.45
Redundancy	25.4	25.8
CC(1/2)	0.998	0.736

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	0.1M Tris pH8.5, 20% PEG 8000, 0.2M MgCl2