8CHV
Crystal structure of human PURA (fragment Glu57-Glu212, PUR repeat I and II) R140P mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-26 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.307, 57.573, 84.879 |
| Unit cell angles | 90.00, 102.33, 90.00 |
Refinement procedure
| Resolution | 47.290 - 2.150 |
| R-factor | 0.2087 |
| Rwork | 0.205 |
| R-free | 0.28210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.455 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Number of reflections | 32410 | 5012 |
| <I/σ(I)> | 7.6 | |
| Completeness [%] | 99.4 | 97.7 |
| Redundancy | 3.5 | |
| CC(1/2) | 0.997 | 0.583 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | SMALL TUBES | 292 | 200 mM NaCl, 20 mM HEPES, 2 mM DTT |
