8CHO

CRYSTAL STRUCTURE OF DELTA5-3-KETOSTEROID ISOMERASE FROM PSEUDOMONAS TESTOSTERONI

Experimental procedure

Spacegroup name	P 65 2 2
Unit cell lengths	61.680, 61.680, 143.600
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 2.300
R-factor	0.205
Rwork	0.205
R-free	0.27100
RMSD bond length	0.012 *
RMSD bond angle	1.615 *
Phasing software	X-PLOR (3.0)
Refinement software	X-PLOR (3.2)

Data quality characteristics

	Overall
High resolution limit [Å]	2.300 *
Completeness [%]	95.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *	12 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	1.3 (M)
2	1	reservoir	PEG400	3-5 (%)
3	1	reservoir	HEPES	0.1 (M)
4	1	drop	protein	5 (mg/ml)