8CHE
TLT-1 binding Fab of the bispecific antibody HMB-001 in complex with the TLT-1 stalk peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX IV BEAMLINE BioMAX |
| Synchrotron site | MAX IV |
| Beamline | BioMAX |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-28 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 |
| Unit cell lengths | 53.234, 65.377, 67.145 |
| Unit cell angles | 91.88, 91.72, 92.89 |
Refinement procedure
| Resolution | 28.460 - 1.490 |
| R-factor | 0.1572 |
| Rwork | 0.157 |
| R-free | 0.17400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.955 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.460 | 1.543 |
| High resolution limit [Å] | 1.490 | 1.490 |
| Rmerge | 0.052 | 0.593 |
| Rmeas | 0.074 | 0.838 |
| Rpim | 0.052 | 0.593 |
| Number of reflections | 140068 | 25651 |
| <I/σ(I)> | 9.03 | 1.37 |
| Completeness [%] | 91.0 | 62.1 |
| Redundancy | 1.9 | 1.9 |
| CC(1/2) | 0.994 | 0.068 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 291 | 1M lithium chloride, 20% (w/v) PEG 6000, 100 mM citric acid/NaOH, pH 4.0 |
