8CHE

TLT-1 binding Fab of the bispecific antibody HMB-001 in complex with the TLT-1 stalk peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX IV BEAMLINE BioMAX
Synchrotron site	MAX IV
Beamline	BioMAX
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-28
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.98
Spacegroup name	P 1
Unit cell lengths	53.234, 65.377, 67.145
Unit cell angles	91.88, 91.72, 92.89

Refinement procedure

Resolution	28.460 - 1.490
R-factor	0.1572
Rwork	0.157
R-free	0.17400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	0.955
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.460	1.543
High resolution limit [Å]	1.490	1.490
Rmerge	0.052	0.593
Rmeas	0.074	0.838
Rpim	0.052	0.593
Number of reflections	140068	25651
<I/σ(I)>	9.03	1.37
Completeness [%]	91.0	62.1
Redundancy	1.9	1.9
CC(1/2)	0.994	0.068

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	291	1M lithium chloride, 20% (w/v) PEG 6000, 100 mM citric acid/NaOH, pH 4.0