8CH9
Crystal structure of arsenite oxidase from Alcaligenes faecalis (Af Aio) bound to arsenic oxyanion
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-11-04 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 |
Unit cell lengths | 90.288, 109.950, 117.424 |
Unit cell angles | 98.33, 89.97, 96.58 |
Refinement procedure
Resolution | 65.470 - 1.430 |
R-factor | 0.16811 |
Rwork | 0.167 |
R-free | 0.19459 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.015 |
RMSD bond angle | 2.171 |
Data reduction software | autoPROC |
Data scaling software | autoPROC |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 65.474 | 1.560 |
High resolution limit [Å] | 1.430 | 1.430 |
Number of reflections | 594307 | 29716 |
<I/σ(I)> | 9.4 | |
Completeness [%] | 75.0 | |
Redundancy | 3.5 | |
CC(1/2) | 0.997 | 0.589 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 10% v/v PEG 6000, 0.01 M magnesium chloride |