8CH0
Crystal structure of an 8-repeat consensus TPR superhelix with Gadolinium.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-10-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.711822 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.228, 57.730, 76.289 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.030 - 1.750 |
| R-factor | 0.18005 |
| Rwork | 0.179 |
| R-free | 0.20984 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.774 |
| Data reduction software | autoPROC |
| Data scaling software | XSCALE |
| Phasing software | MOLREP (11.7.02) |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.730 | 1.840 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmeas | 0.106 | 0.687 |
| Rpim | 0.031 | 0.207 |
| Total number of observations | 234292 | 32240 |
| Number of reflections | 20384 | 2930 |
| <I/σ(I)> | 13.2 | 3.1 |
| Completeness [%] | 100.0 | |
| Redundancy | 11.5 | 11 |
| CC(1/2) | 0.997 | 0.958 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 292 | 5mM GdCl3 100 mM Tris HCl 30% MPD |
