8CGM

Structure of the lipoprotein transporter LolA from Porphyromonas gingivalis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9762
Spacegroup name	P 21 21 21
Unit cell lengths	48.810, 76.463, 99.436
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.720 - 1.700
R-factor	0.1895
Rwork	0.188
R-free	0.22120
Structure solution method	SAD
RMSD bond length	0.014
RMSD bond angle	1.559
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	Auto-Rickshaw
Refinement software	PHENIX (1.17)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.720	1.761
High resolution limit [Å]	1.700	1.700
Rmerge	0.134	1.299
Rmeas		1.349
Rpim	0.038	0.359
Number of reflections	41710	4084
<I/σ(I)>	20.3	3.6
Completeness [%]	100.0	100
Redundancy	25.4	26.9
CC(1/2)	0.999	0.865

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	0.2 M Na formate, 0.2 M NH4acetate, 0.2 M Na citrate tribasic, 0.2 M Na/K tartrate, 0.1 M imidazole/MES pH 6.5, 40% PEG500mme, 20% PEG20000