8CEK

Succinyl-CoA Reductase from Clostridium kluyveri (SucD) with NADPH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	80
Detector technology	PIXEL
Collection date	2019-03-30
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97620
Spacegroup name	I 2 2 2
Unit cell lengths	140.046, 190.828, 190.907
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.660 - 2.150
R-factor	0.1983
Rwork	0.198
R-free	0.22190
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.585
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.20.1-4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.440	2.150
High resolution limit [Å]	2.040	2.040
Rmeas	0.215	2.931
Rpim	0.059	0.966
Total number of observations	2045343	169254
Number of reflections	159239	21156
<I/σ(I)>	8.5	0.6
Completeness [%]	98.6
Redundancy	12.8	8
CC(1/2)	0.998	0.329

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	288.15	45 % w/v Pentaerythritol Propoxylate (5/4 PO/OH) 100 mM MES; pH 6.5 400 mM Potassium chloride