8CDY

N-terminal domain of human apolipoprotein E

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-04-23
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	0.999
Spacegroup name	P 21 21 21
Unit cell lengths	45.567, 53.025, 72.577
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.591 - 1.900
R-factor	0.2131
Rwork	0.212
R-free	0.23070
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.695
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1-4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.820	1.940
High resolution limit [Å]	1.900	1.900
Number of reflections	13501	563
<I/σ(I)>	33.8
Completeness [%]	93.7
Redundancy	11.1
CC(1/2)	1.000	0.637

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293.15	HEPES, PEG 6000