8CD8
Ulilysin - C269A with AEBSF complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-06-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97866 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 50.130, 124.660, 86.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 62.330 - 1.650 |
| R-factor | 0.2077 |
| Rwork | 0.207 |
| R-free | 0.24290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.901 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX (1.18.2_3874) |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.900 | 1.650 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmeas | 0.125 | 0.125 |
| Number of reflections | 66338 | 66008 |
| <I/σ(I)> | 11.8 | |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100 mM MES pH 6.5, 200 mM calcium chloride, 18% polyethylene glycol 8000 |
