8CB8
The Transcriptional Regulator PrfA from Listeria Monocytogenes in complex with tetrapeptide Ser-Thr-Leu-Leu
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX IV BEAMLINE BioMAX |
Synchrotron site | MAX IV |
Beamline | BioMAX |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-02-28 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9184 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 47.952, 86.133, 115.217 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.890 - 2.250 |
R-factor | 0.2269 |
Rwork | 0.224 |
R-free | 0.28720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6hck |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.000 | 2.330 |
High resolution limit [Å] | 2.250 | 2.250 |
Rpim | 0.106 | 1.797 |
Number of reflections | 23371 | 2282 |
<I/σ(I)> | 9.2 | 1.6 |
Completeness [%] | 100.0 | 100 |
Redundancy | 13.4 | 3.5 |
CC(1/2) | 0.996 | 0.671 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291 | Protein: 3.1-6.6 mg mL-1, 20 mM NaP pH 6.5, 200 mM NaCl was mixed with peptides (0.5-2.5% DMSO, 1-4 mM DTT) in ratio 1:10. Well solution: 100 mM Nacitrate pH 5.1-5.7, 16-26% PEG4000. Dropsize 1:1 microL Cryoprotection: 35% PEG4000 |