8C7U
Transcriptional pleiotropic repressor CodY from Enterococcus faecalis in complex with Leu and a 30-bp DNA fragment encompassing two overlapping binding sites
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-09-14 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 0.9116 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 86.747, 98.692, 168.363 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.790 - 3.150 |
| R-factor | 0.2498 |
| Rwork | 0.248 |
| R-free | 0.28470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.762 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (phenix1.17.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.790 | 3.260 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Rpim | 0.073 | 1.010 |
| Number of reflections | 25663 | 2516 |
| <I/σ(I)> | 8.5 | 1 |
| Completeness [%] | 100.0 | |
| Redundancy | 13.2 | |
| CC(1/2) | 0.997 | 0.441 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | Protein,200 microM in 20 mM TrisCl pH 8, 150 mM NaCl,4 mM Leu, 60 microM DNA Well, 0.01 M CoCl2, 0.01 M MnCl2, 0.1 M NaAc pH 4.6,1 M 1,6-Hexanediol |
