8C7T

Unliganded transcriptional pleiotropic repressor CodY from Enterococcus faecalis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9763
Spacegroup name	P 21 21 21
Unit cell lengths	32.988, 171.241, 215.236
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.870 - 2.210
R-factor	0.2376
Rwork	0.236
R-free	0.27340
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.291
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.870	2.290
High resolution limit [Å]	2.210	2.210
Rpim	0.077	0.876
Number of reflections	62752	6079
<I/σ(I)>	7.3	1.1
Completeness [%]	99.9
Redundancy	6.4
CC(1/2)	0.995	0.501

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	Protein, 600 microM in 20 mM TrisCl pH 8, 450 mM NaCl Well, 200 mM ammoniumhydrogenphosphate, 2.5% EtOH, 23-25% PEG3350