8C6V

The RNA silencing suppressor P8 from High-Plains Wheat Mosaic Virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.9
Spacegroup name	P 1 21 1
Unit cell lengths	36.960, 152.390, 65.400
Unit cell angles	90.00, 101.01, 90.00

Refinement procedure

Resolution	49.090 - 2.150
R-factor	0.2015
Rwork	0.200
R-free	0.23360
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.469
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	AutoSol
Refinement software	PHENIX (v1.20.1-4487-000)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	30.000	2.200
High resolution limit [Å]	2.150	20.000	2.150
Rmerge	0.073	0.050	1.914
Rmeas	0.075	0.051	1.982
Number of reflections	36581	37	2307
<I/σ(I)>	24.45
Completeness [%]	94.9
Redundancy	20.1
CC(1/2)	1.000	1.000	0.649

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	289	22% PEG 3350 0.1M Tris pH 7.5 0.2M NaCl 0.4M Guanidine HCL