8C67
Crystal structure of Ab25 Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX IV BEAMLINE BioMAX |
| Synchrotron site | MAX IV |
| Beamline | BioMAX |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-02-25 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 86.897, 71.890, 146.014 |
| Unit cell angles | 90.00, 90.10, 90.00 |
Refinement procedure
| Resolution | 44.160 - 1.880 |
| R-factor | 0.2078 |
| Rwork | 0.205 |
| R-free | 0.25500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6jc2 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.089 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.670 | 1.950 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Number of reflections | 146332 | 14268 |
| <I/σ(I)> | 11.7 | 1.9 |
| Completeness [%] | 99.9 | |
| Redundancy | 6.94 | |
| CC(1/2) | 0.998 | 0.835 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293.15 | 27.50% (w/v) PEG 8000, 0.1 M CHES pH 8.5 |
