8C4Z
ProteinT protease, inactive mutant S224A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.353, 80.559, 49.723 |
| Unit cell angles | 90.00, 96.97, 90.00 |
Refinement procedure
| Resolution | 49.360 - 1.470 |
| R-factor | 0.1413 |
| Rwork | 0.139 |
| R-free | 0.17860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.854 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (v1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.560 | 1.490 |
| High resolution limit [Å] | 1.470 | 1.470 |
| Rmerge | 0.070 | 0.680 |
| Number of reflections | 50693 | 2568 |
| <I/σ(I)> | 11.36 | |
| Completeness [%] | 85.8 | |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 285 | 10% PEG 8K 0.2M MgAcetate 0.1 Sodium Cacodilate pH 6.5 |
