8C4X
PAM Protease
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9724 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.482, 64.175, 62.959 |
| Unit cell angles | 90.00, 96.16, 90.00 |
Refinement procedure
| Resolution | 62.600 - 1.520 |
| R-factor | 0.1514 |
| Rwork | 0.150 |
| R-free | 0.17650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.920 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (v1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.600 | 1.570 |
| High resolution limit [Å] | 1.520 | 1.520 |
| Rmerge | 0.090 | 0.520 |
| Number of reflections | 48618 | 18284 |
| <I/σ(I)> | 9.38 | |
| Completeness [%] | 98.2 | |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 285 | 40% MPD 0.1M Sodium Citrate pH 5.6 |
