8C3H

Cereblon isoform 4 from Magnetospirillum gryphiswaldense in complex a long aspartimide degron peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-03
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9998
Spacegroup name	P 21 21 21
Unit cell lengths	56.256, 58.821, 88.858
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.530 - 1.710
Rwork	0.179
R-free	0.21040
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	4v2y
RMSD bond length	0.013
RMSD bond angle	1.769
Data reduction software	XDS
Data scaling software	XDS
Refinement software	REFMAC (5.8.0266)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.530	1.810
High resolution limit [Å]	1.710	1.710
Rmerge	0.082	1.629
Number of reflections	32554	5215
<I/σ(I)>	14.3	1.15
Completeness [%]	99.7	98.8
Redundancy	12.84	12.42
CC(1/2)	1.000	0.701

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		274	0.5 M (NH4)H2PO4