8C3A
Crystal structure of ailanthone bound to the Candida albicans 80S ribosome
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-03-19 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 298.960, 294.090, 450.210 |
| Unit cell angles | 90.00, 100.12, 90.00 |
Refinement procedure
| Resolution | 227.460 - 3.000 |
| R-factor | 0.2682 |
| Rwork | 0.268 |
| R-free | 0.29950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.783 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX (19.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 227.500 | 3.100 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmeas | 0.759 | |
| Number of reflections | 1514598 | 73124 |
| <I/σ(I)> | 4.81 | |
| Completeness [%] | 99.4 | |
| Redundancy | 21.6 | |
| CC(1/2) | 0.994 | 0.040 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | PEG 20000, potassium acetate, potassium thiocyanate, magnesium acetate, bis-tris, spermidine |
