8C26
ParDE2 toxin-antitoxin complex from Mycobacterium tuberculosis (rv2142A-rv2142c)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-10-18 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 68.074, 68.074, 197.049 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.590 - 2.350 |
| R-factor | 0.2575 |
| Rwork | 0.254 |
| R-free | 0.28840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.995 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.590 | 2.429 |
| High resolution limit [Å] | 2.345 | 2.345 |
| Number of reflections | 7244 | 349 |
| <I/σ(I)> | 7.5 | |
| Completeness [%] | 94.1 | |
| Redundancy | 19.8 | |
| CC(1/2) | 1.000 | 0.600 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 15 % PEG 3350 0.1 M MES pH 6.2 |
