8C0T

NRS 1.2: Fluorescent Sensors for Imaging Interstitial Calcium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	54.332, 62.854, 95.709
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.850 - 1.280
R-factor	0.1898
Rwork	0.189
R-free	0.20220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5mwc
RMSD bond length	0.008
RMSD bond angle	1.053
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.855	1.325
High resolution limit [Å]	1.279	1.279
Number of reflections	85049	90739
<I/σ(I)>	15.6
Completeness [%]	100.0
Redundancy	12.3
CC(1/2)	0.999	0.389

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		292	Ammonium sulfate 0.2M PEG 400 30%