8BZR
UFC1-UFM1 conjugate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-04-08 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.97950 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 56.005, 56.427, 86.958 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.334 - 1.781 |
| Rwork | 0.182 |
| R-free | 0.22640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3evx |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.726 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0403) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.330 | 1.810 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Number of reflections | 26839 | 1192 |
| <I/σ(I)> | 9.2 | 0.5 |
| Completeness [%] | 99.1 | 88.6 |
| Redundancy | 12.5 | 7.5 |
| CC(1/2) | 0.998 | 0.270 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 292 | 30% v/v PEG400, 0.1M Tris, 0.2M Na citrate |
