8BZM
FOXK1-ELF1-heterodimer bound to DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-30 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.6199 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.181, 105.868, 68.194 |
| Unit cell angles | 90.00, 112.94, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.690 |
| R-factor | 0.27352 |
| Rwork | 0.271 |
| R-free | 0.32360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.693 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.490 | 2.840 |
| High resolution limit [Å] | 2.690 | 2.690 |
| Rmerge | 0.200 | 1.999 |
| Rmeas | 0.232 | 2.310 |
| Rpim | 0.116 | 1.147 |
| Total number of observations | 92854 | 13710 |
| Number of reflections | 23997 | 3493 |
| <I/σ(I)> | 3.9 | 0.4 |
| Completeness [%] | 99.8 | |
| Redundancy | 3.9 | 3.9 |
| CC(1/2) | 0.991 | 0.399 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 293 | PEG MME 3350, PEG 200, magnesium chloride, HEPES |
