8BZB

co-soak stabilizers for ERa - 14-3-3 interaction (884_AZ275)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	RIGAKU MICROMAX-003
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-23
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.541870
Spacegroup name	C 2 2 21
Unit cell lengths	81.905, 112.491, 62.402
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.950 - 1.700
R-factor	0.1649
Rwork	0.163
R-free	0.19330
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.036
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.19.2-4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	66.210	1.730
High resolution limit [Å]	1.700	1.700
Number of reflections	31123	1174
<I/σ(I)>	28.7
Completeness [%]	97.0
Redundancy	5.8
CC(1/2)	0.999	0.967

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.095 M HEPES (pH 7.1), PEG400 (24% (v/v)), 0.19 M CaCl2 and 5% (v/v) Glycerol