8BZ6

Crystal structure of the L. monocytogenes RmlT in complex with UDP-glucose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.978565
Spacegroup name	P 1 21 1
Unit cell lengths	85.523, 291.057, 89.824
Unit cell angles	90.00, 100.08, 90.00

Refinement procedure

Resolution	44.220 - 2.400
R-factor	0.1968
Rwork	0.195
R-free	0.23510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8bz4
RMSD bond length	0.004
RMSD bond angle	0.581
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.340	2.440
High resolution limit [Å]	2.400	2.400
Rmerge	0.103	1.560
Rmeas	0.111	1.678
Rpim	0.041	0.615
Number of reflections	166303	8154
<I/σ(I)>	12.2	1.3
Completeness [%]	99.1	97.7
Redundancy	7.2	7.3
CC(1/2)	0.998	0.620

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Tris-HCl pH 8.5 0.2 M Sodium acetate 15% (w/v) PEG 4,000