8BZ2
Crystal structure of outer membrane attachment porin OmpM1 SLH domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-07-29 |
| Detector | DECTRIS EIGER2 S 16M |
| Wavelength(s) | 0.8984 |
| Spacegroup name | H 3 |
| Unit cell lengths | 118.710, 118.710, 47.240 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.780 - 1.700 |
| R-factor | 0.225 |
| Rwork | 0.224 |
| R-free | 0.24900 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.219 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | Arcimboldo |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.780 | 1.600 |
| High resolution limit [Å] | 1.570 | 1.570 |
| Rmerge | 0.049 | 1.720 |
| Rpim | 0.017 | 0.884 |
| Number of reflections | 34489 | 1590 |
| <I/σ(I)> | 17.1 | 0.7 |
| Completeness [%] | 99.5 | 92.9 |
| Redundancy | 9 | 4.7 |
| CC(1/2) | 1.000 | 0.230 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 3.5 | 293 | 0.1 M citric acid pH 3.5 2.0 M ammonium sulphate |
