8BYK

The structure of MadC from Clostridium maddingley reveals new insights into class I lanthipeptide cyclases

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X13
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X13
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-08-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9763
Spacegroup name	P 21 21 21
Unit cell lengths	54.140, 66.119, 117.258
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.890 - 1.700
Rwork	0.190
R-free	0.22000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.027
RMSD bond angle	2.209
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0131)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.890	1.761
High resolution limit [Å]	1.700	1.700
Number of reflections	47068	4591
<I/σ(I)>	29.63
Completeness [%]	100.0
Redundancy	13
CC(1/2)	0.999	0.995

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		298	lithium chloride and MPD