8BX1

Oct4/Sox2 protein:DNA complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97624
Spacegroup name	P 21 21 21
Unit cell lengths	51.199, 65.892, 126.920
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	58.480 - 2.500
R-factor	0.25414
Rwork	0.253
R-free	0.27410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ht5
RMSD bond length	0.012
RMSD bond angle	2.375
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	MOLREP (11.7.03)
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	126.920	2.600
High resolution limit [Å]	2.500	2.500
Rmerge	0.091	1.280
Number of reflections	15480	1711
<I/σ(I)>	16.5	2
Completeness [%]	99.7
Redundancy	11.4
CC(1/2)	0.999	0.755

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	277	0.1M sodium acetate, 12% (v/v) PEG 4000