8BWD
Crystal structure of human Twisted gastrulation protein homolog 1 (TWSG1), crystal form 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-25 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 97.993, 97.993, 187.616 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.430 - 2.630 |
| R-factor | 0.2616 |
| Rwork | 0.259 |
| R-free | 0.30540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.652 |
| Data reduction software | XDS |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 84.864 | 2.999 |
| High resolution limit [Å] | 2.627 | 2.627 |
| Rmerge | 0.078 | 1.603 |
| Rmeas | 0.080 | 1.648 |
| Rpim | 0.019 | 0.377 |
| Number of reflections | 8855 | 443 |
| <I/σ(I)> | 22 | 1.9 |
| Completeness [%] | 53.0 | 8.4 |
| Redundancy | 18.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.85 M LiCl, 17.1% w/v PEG 6000, 85 mM HEPES pH 7.0, 3.6% 1,1,1,3,3,3-hexafluoro-2-propanol |
