8BW1
Yeast 20S proteasome in complex with an engineered fellutamide derivative (C14QAL)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-08-21 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 134.970, 300.870, 143.980 |
| Unit cell angles | 90.00, 112.78, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.250 |
| R-factor | 0.1764 |
| Rwork | 0.174 |
| R-free | 0.21218 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ryp |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.204 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.350 |
| High resolution limit [Å] | 3.250 | 3.250 |
| Rmerge | 0.105 | 0.654 |
| Number of reflections | 157953 | 13542 |
| <I/σ(I)> | 11.1 | 2.4 |
| Completeness [%] | 95.1 | 93.7 |
| Redundancy | 3 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 293 | 100 mM MES, 20 mM MgAc2, 11% MPD |
