8BVX

Structure of the mouse 8-oxoguanine DNA Glycosylase mOGG1 in complex with ligand TH13264

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX IV BEAMLINE BioMAX
Synchrotron site	MAX IV
Beamline	BioMAX
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9762
Spacegroup name	P 21 21 21
Unit cell lengths	81.589, 81.874, 169.954
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	58.850 - 2.350
Rwork	0.263
R-free	0.30240
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.659
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.850	2.430
High resolution limit [Å]	2.350	2.350
Number of reflections	48282	4378
<I/σ(I)>	12.1
Completeness [%]	100.0
Redundancy	13.4
CC(1/2)	0.999	0.576

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	10 % w/v PEG4000, 20 % glycerol, 0.03 M ethylene glycol, 0.1 M MOPS/HEPES-Na pH 7.5