8BU0

Crystal structure of an 8 repeat consensus TPR superhelix with calcium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9791
Spacegroup name	P 32 2 1
Unit cell lengths	67.428, 67.428, 72.613
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.550 - 1.400
R-factor	0.18328
Rwork	0.183
R-free	0.19734
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.720
Data reduction software	MOSFLM (7.4.0)
Data scaling software	SCALA (3.3.22)
Phasing software	MOLREP
Refinement software	REFMAC (5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.400	1.480
High resolution limit [Å]	1.400	1.400
Rmeas	0.080	1.905
Rpim	0.018	0.441
Total number of observations	676928	98822
Number of reflections	37860	5477
<I/σ(I)>	14.2	1.8
Completeness [%]	99.5
Redundancy	17.9	18
CC(1/2)	0.999	0.631

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	292	100 mM NaOAc, pH 5.5, 20 mM CaCl2, 30% MPD