8BTY
Structure of the active form of ScpB, the C5a-peptidase from Streptococcus agalactiae.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97950 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 167.152, 167.152, 141.485 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.840 - 1.700 |
| R-factor | 0.1895 |
| Rwork | 0.188 |
| R-free | 0.21610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3eif |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.773 |
| Data reduction software | XDS (January 31, 2020) |
| Phasing software | PHASER (2.7.17) |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.253 | 1.761 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmeas | 0.212 | 5.659 |
| Rpim | 0.033 | 0.895 |
| Number of reflections | 127120 | 12428 |
| <I/σ(I)> | 16.63 | 0.34 |
| Completeness [%] | 99.9 | 99.01 |
| Redundancy | 40 | 39.6 |
| CC(1/2) | 1.000 | 0.237 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 293 | 2.5 M ammonium sulfate, 0.1 M Hepes/KOH pH 7.8 |
