8BT9

Crystal structure of SlpA domain II (aa 201-310), domain that is involved in the self-assembly and dimerization of the S-layer from Lactobacillus acidophilus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P11
Synchrotron site	PETRA III, DESY
Beamline	P11
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.033230
Spacegroup name	P 32 2 1
Unit cell lengths	137.690, 137.690, 51.301
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.124 - 2.100
Rwork	0.189
R-free	0.22210
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.498
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.124	2.175
High resolution limit [Å]	2.100	2.100
Rmerge	0.012	0.485
Number of reflections	32853	3272
<I/σ(I)>	23.76	1.57
Completeness [%]	100.0	99.97
Redundancy	2	2
CC(1/2)	1.000	0.909

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	Condition: 1.0 M Sodium citrate tribasic dihydrate, 0.1 M MES, pH 6.5 Protein: 10 mg/ml in 150 mM NaCl, 25 mM HEPES, pH 7.0 0.5 ul protein mixed with 0.5 ul condition