8BSO
crystal structure of antibody Fab with SiaLac-amidine-Lys
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-01-24 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.99985 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 117.688, 130.251, 77.693 |
| Unit cell angles | 90.00, 123.77, 90.00 |
Refinement procedure
| Resolution | 64.580 - 1.920 |
| R-factor | 0.1817 |
| Rwork | 0.179 |
| R-free | 0.22030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ug7 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.146 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.580 | 1.989 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmerge | 0.105 | 0.804 |
| Number of reflections | 74047 | 7398 |
| <I/σ(I)> | 8.85 | 0.9 |
| Completeness [%] | 99.8 | 99.2 |
| Redundancy | 3.7 | |
| CC(1/2) | 0.305 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | KSCN, PEG MME 2000 |
