8BRX
Escherichia coli methionyl-tRNA synthetase mutant L13C,I297C complexed with beta-3-methionine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-08 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 78.660, 45.510, 86.430 |
| Unit cell angles | 90.00, 107.51, 90.00 |
Refinement procedure
| Resolution | 41.820 - 1.540 |
| R-factor | 0.1664 |
| Rwork | 0.165 |
| R-free | 0.19830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.637 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.630 |
| High resolution limit [Å] | 1.530 | 1.530 |
| Rmeas | 0.109 | 1.275 |
| Number of reflections | 86690 | 13088 |
| <I/σ(I)> | 14.36 | 1.16 |
| Completeness [%] | 98.6 | 92.4 |
| Redundancy | 6.6 | 6.4 |
| CC(1/2) | 0.998 | 0.502 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 1.08 M ammonium citrate, 30mM potassium phosphate |
