8BQG
W-formate dehydrogenase from Desulfovibrio vulgaris - Soaking with Formate 1 min
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-03-01 |
| Detector | DECTRIS EIGER2 S 16M |
| Wavelength(s) | 0.8856 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.537, 122.778, 148.985 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 94.930 - 1.946 |
| Rwork | 0.193 |
| R-free | 0.24270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6sdr |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.618 |
| Data reduction software | autoPROC |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 94.930 | 2.070 |
| High resolution limit [Å] | 1.946 | 1.950 |
| Number of reflections | 73334 | 3667 |
| <I/σ(I)> | 9.2 | |
| Completeness [%] | 92.0 | |
| Redundancy | 4.33 | |
| CC(1/2) | 0.997 | 0.435 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 24% PEG 3350, 0.1M Tris-HCl pH 8.0, 1M LiCl |
