8BP9
Structure of E. coli Class 2 L-asparaginase EcAIII, mutant M200W (crystal M200W#2)
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | Agilent SuperNova |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2022-10-25 |
| Detector | AGILENT ATLAS CCD |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.974, 74.475, 146.787 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 73.400 - 1.700 |
| Rwork | 0.193 |
| R-free | 0.22840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zal |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.598 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.400 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.082 | 0.378 |
| Rmeas | 0.094 | 0.455 |
| Number of reflections | 61686 | 3153 |
| <I/σ(I)> | 9.9 | 2.5 |
| Completeness [%] | 99.1 | 97.8 |
| Redundancy | 4 | 3.1 |
| CC(1/2) | 0.995 | 0.749 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 20-30% PEG4000, 10-15%PEG400,0.2M MgCl2 in 100 mM Tris-HCl pH 8.5 |
