8BP4

Crystal structure of Trichoplax Scribble PDZ2 domain in complex with Trichoplax Vangl peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.95372
Spacegroup name	P 1 21 1
Unit cell lengths	42.303, 43.387, 71.316
Unit cell angles	90.00, 91.08, 90.00

Refinement procedure

Resolution	42.300 - 1.150
R-factor	0.1799
Rwork	0.178
R-free	0.21080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7jo7
RMSD bond length	0.018
RMSD bond angle	1.459
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.390	1.170
High resolution limit [Å]	1.150	1.150
Rmerge	0.035	1.328
Number of reflections	91308	4253
<I/σ(I)>	7.5
Completeness [%]	99.5
Redundancy	3.6
CC(1/2)	0.999	0.337

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.2 M ammonium sulphate, PEG 4000