8BOR

Photosensory module from DrBphP without PHY tongue

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-02
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.07225
Spacegroup name	P 1 21 1
Unit cell lengths	119.630, 64.590, 131.040
Unit cell angles	90.00, 91.55, 90.00

Refinement procedure

Resolution	45.990 - 2.300
R-factor	0.23667
Rwork	0.235
R-free	0.26819
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5k5b
RMSD bond length	0.004
RMSD bond angle	0.989
Data reduction software	XDS (Feb 5, 2021)
Data scaling software	XSCALE (Feb 5, 2021)
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.360
High resolution limit [Å]	2.300	10.000	2.300
Rmerge	0.152	0.064	1.496
Rmeas	0.181	0.078	1.792
Number of reflections	88343	1154	6483
<I/σ(I)>	5.4
Completeness [%]	98.8	98.5	99.2
Redundancy	3.292		3.259
CC(1/2)	0.989	0.993	0.325

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	12% polyethylene glycol (PEG) 3350, 200 mM ammonium acetate, 5% fructose, 5% glucose, and 100 mM sodium citrate pH5.6