8BNY
Structure of the tetramerization domain of pLS20 conjugation repressor Rco
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-11-01 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9793 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 35.328, 36.182, 109.532 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.356 - 1.429 |
R-factor | 0.234 |
Rwork | 0.233 |
R-free | 0.24910 |
Structure solution method | AB INITIO PHASING |
RMSD bond length | 0.006 |
RMSD bond angle | 0.724 |
Phasing software | Arcimboldo |
Refinement software | PHENIX (1.12-2829) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.360 | 1.433 |
High resolution limit [Å] | 1.409 | 1.409 |
Rmeas | 0.044 | 0.780 |
Number of reflections | 18842 | 943 |
<I/σ(I)> | 14.5 | 1.7 |
Completeness [%] | 91.6 | |
Redundancy | 5.1 | |
CC(1/2) | 0.999 | 0.512 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291.15 | 0.1 M Hepes pH 7.5, 28% (v/v) PEG 600 |