8BNT
The DH domain of ARHGEF2 bound to RhoA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-09-15 |
| Detector | DECTRIS EIGER2 XE 9M |
| Wavelength(s) | 0.9212 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 71.432, 71.432, 196.527 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 57.845 - 1.400 |
| Rwork | 0.130 |
| R-free | 0.17510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4d0n |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.640 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.430 | 1.420 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rpim | 0.072 | 3.132 |
| Number of reflections | 101036 | 4914 |
| <I/σ(I)> | 10.3 | 0.7 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 45.8 | 22.9 |
| CC(1/2) | 0.999 | 0.403 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 2.6 M sodium formate, 100 mM Tris |
