8BN2

Crystal structure of the ligand-binding domain (LBD) of human iGluR Delta-1 (GluD1) in complex with D-Serine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-16
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.999
Spacegroup name	P 21 21 21
Unit cell lengths	45.209, 66.845, 175.901
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.450 - 1.630
R-factor	0.1832
Rwork	0.182
R-free	0.21430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2v3u
RMSD bond length	0.006
RMSD bond angle	0.851
Data reduction software	XDS (Feb 5, 2021 (BUILT 20210323))
Data scaling software	Aimless (0.7.7)
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0270)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	87.950	1.720
High resolution limit [Å]	1.630	1.630
Rmerge	0.060	1.190
Rpim	0.024	0.496
Number of reflections	59381	2970
<I/σ(I)>	14.1	1.5
Completeness [%]	94.2	51.3
Redundancy	7	6.6
CC(1/2)	0.998	0.529

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	294	30% (w/v) PEG 4000, 100 mM Tris pH 7.5, 200 mM CaCl2