8BM8
Crystal Structure of Ephrin A2 (EphA2) Receptor Protein Kinase with Compound 11
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-12-01 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.540, 106.430, 40.250 |
| Unit cell angles | 90.00, 108.44, 90.00 |
Refinement procedure
| Resolution | 38.180 - 1.680 |
| R-factor | 0.1915 |
| Rwork | 0.190 |
| R-free | 0.22490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6q7d |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.989 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.180 | 1.780 |
| High resolution limit [Å] | 1.680 | 1.680 |
| Rmeas | 0.141 | |
| Number of reflections | 28709 | 4461 |
| <I/σ(I)> | 7.75 | |
| Completeness [%] | 96.5 | 92.9 |
| Redundancy | 3.32 | 3.17 |
| CC(1/2) | 0.995 | 0.414 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 291 | 37,5 % MPD_PEG1000_PEG3350, 100 mM Morpheus Amino Acids Mix, 100 mM Bis-Tris pH 6.0 |
